Nutrient Broth

Nutrient Broth is used for the cultivation of a wide variety of microorganisms in a laboratory setting. Nutrient Broth is not intended for use in the diagnosis of disease or other conditions in humans.

In the early 1900's, the American Public Health Association (APHA) suggested the formula of Nutrient Agar as a standard culture medium used in water testing. Nutrient Broth is the same formulation as Nutrient Agar, only Agar has been omitted.

Nutrient Broth is used as a pre-enrichment medium when testing certain foods and dairy products for Salmonella spp. In dried or processed foods, salmonellae may be sublethally injured and in low numbers. The presence of other bacteria and food sample components may hinder growth and recovery of Salmonella spp. Pre-enrichment in a nonselective medium such as Nutrient Broth allows for cell damage repair, dilutes toxic or inhibitory substances, and provides a nutritional advantage to Salmonella over other bacteria.

Nutrient Broth is included in many standard methods procedures for testing food, dairy products, and other materials.


Formula Liter
Enzymatic Digest of Gelatin 5 g
Beef Extract 3 g

Final pH: 6.8 ± 0.2 at 25°C
Formula may be adjusted and/or supplemented as required to meet performance specifications.


  1. Dissolve 8 g of the medium in one liter of purified water.
  2. Mix thoroughly.
  3. Autoclave at 121°C for 15 minutes.

Order Details

Product # Product Description
7146A Nutrient Broth, 500 g
7146T Nutrient Broth, 5 kg