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Oxford Listeria Agar Base

Oxford Listeria Agar Base is used with antimicrobics for the selective isolation of Listeria spp. in a laboratory setting. Oxford Listeria Agar Base is not intended for use in the diagnosis of disease or other conditions in humans.

Listeria monocytogenes, described first in 1926 by Murray, Webb, and Swann, is an extensive problem food industries. Epidemiological evidence from outbreaks of listeriosis has indicated that the principle route of transmission is via the consumption of foodstuffs contaminated with Listeria monocytogenes. Implicated vehicles of transmission included turkey frankfurters, coleslaw, pasteurized milk, Mexican style cheese, and pate. Listeria spp. are ubiquitous in nature, being present in a wide range of unprocessed foods as well as in soil, sewage, and river water.

Oxford Listeria Agar Base is prepared according to the formulation of Curtis et al. Listeria spp. grow over a pH range of 5.0 – 9.6, and survive in food products with pH levels outside these parameters.

 

Formula Liter
Columbia Blood Agar Base 39 g
Esculin 1 g
Ferric Ammonium Citrate 0.5 g
Lithium Chloride 15 g
Agar 2 g

Final pH: 7.0 ± 0.2 at 25°C
Formula may be adjusted and/or supplemented as required to meet performance specifications.

Antimicrobic Supplments / Oxford Listeria Supplement

Acriflavin 5 mg
Cefotetan 2 mg
Colistin Sulfate 20 mg
Cycloheximide 400 mg
Phosphomycin 10 mg

 

Modified Oxford Listeria Supplement

Colistin Sulfate 10 mg
Moxalactam 20 mg

Directions

Oxford Medium Base

  1. Suspend 57.5 g of the medium in one liter of purified water.
  2. Heat with frequent agitation and boil for one minute to completely dissolve the medium.
  3. Autoclave at 121°C for 10 – 15 minutes. Cool to 45 – 50°C.

Oxford Medium

Aseptically add a filtered sterilized aqueous solution of 5 mg acriflavin, 2 mg cefotetan, 20 mg colistin sulfate, 400 mg cycloheximide*, and 10 mg phosphomycin. Note: *Natamycin may be used in place of cycloheximide at 25 mg.

Modified Oxford Medium

Aseptically add a filtered sterilized aqueous solution of 10 mg colistin sulfate and 20 mg moxalactam.

Order Details

Product # Product Description
7428A Oxford Listeria Agar Base, 500 g
7428T Oxford Listeria Agar Base, 5 kg