PALCAM Agar Base

Palcam Agar Base is used with supplements as a selective and differential medium for the detection and isolation of Listeria monocytogenes from foods and environmental samples in a laboratory setting. Palcam Agar Base is not intended for use in the diagnosis of disease or other conditions in humans.

Listeria monocytogenes, described in 1926 by Murray, Webb, and Swann, is a widespread problem in food industries. Evidence from outbreaks of listeriosis indicate the principle route of transmission is via the consumption of foods contaminated with Listeria monocytogenes.

PALCAM Agar Base is based on the formulation of Van Netten et al., while he was investigating the isolation of Listeria spp. from food samples. PALCAM Agar Base is recommended by AFNOR for the detection of Listeria monocytogenes in foods, Health Canada in food and environmental samples, and the International Dairy Federation in milk and milk products.


Formula Liter
Peptone 23 g
Starch 1 g
Sodium Chloride 5 g
Yeast Extract 3 g
Mannitol 10 g
Ferric Ammonium Citrate 0.5 g
Esculin 0.8 g
Dextrose 0.5 g
Lithium Chloride 15 g
Phenol Red 0.08 g
Agar 13 g

Final pH: 7.2 ± 0.2 at 25°C
Formula may be adjusted and/or supplemented as required to meet performance specifications.

PALCAM Supplement / 5 mL

Polymixin B, 5 mg
Acriflavin, 2.5 mg
Ceftazidime, 10 mg
(Each vial supplements 500 mL)


  1. Suspend 72 g of the medium in one liter of purified water.
  2. Heat with frequent agitation and boil for one minute to completely dissolve the medium.
  3. Autoclave at 121°C for 15 minutes. Cool to 45 – 50°C.
  4. Aseptically add 10 mL of PALCAM Supplement (# 7987). One vial will supplement 500 mL of PALCAM Agar Base.
  5. Dispense into sterile Petri dishes.

Order Details

Product # Product Description
7669A PALCAM Agar Base, 500 g
7669T PALCAM Agar Base, 5 kg