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PALCAM Broth

PALCAM Broth is used with supplements for the selective enrichment of Listeria spp. from foods and environmental samples in a laboratory setting. PALCAM Broth is not intended for use in the diagnosis of disease or other conditions in humans.

Listeria monocytogenes, described in 1926 by Murray, Webb, and Swann, is a widespread problem in food industries. Evidence from outbreaks of listeriosis indicate the principle route of transmission is via the consumption of foodstuffs contaminated with Listeria monocytogenes. Implicated vehicles of transmission include turkey frankfurters, coleslaw, pasteurized milk, Mexican style cheese, and pate.

PALCAM Broth is based on the formulation of Van Netten et al. while he was investigating the isolation of Listeria spp. from food samples. PALCAM Broth has been used for testing Listeria from raw milk.

 

Formula Liter
Peptone 23 g
Yeast Extract 5 g
Lithium Chloride 10 g
Ferric Ammonium Citrate 0.5 g
Esculin 0.8 g
Mannitol 5 g
Phenol Red 0.08 g
Lecithin 1 g
Polysorbate 80 2 g

Final pH: 7.4 ± 0.2 at 25°C
Formula may be adjusted and/or supplemented as required to meet performance specifications.

PALCAM Supplement / 5 mL

Polymixin B, 5 mg
Acriflavin, 2.5 mg
Ceftazidime, 10 mg
(Each vial supplements 500 mL)

Directions

  1. Suspend 47 g of the medium in one liter of purified water.
  2. Heat with frequent agitation to completely dissolve the medium.
  3. Autoclave at 121°C for 15 minutes. Cool to 45 – 50°C.
  4. Aseptically add 10 mL of PALCAM Supplement. One vial of PALCAM Supplement will supplement 500 mL of PALCAM Broth. Dispense into sterile tubes.

Order Details

Product # Product Description
7670A PALCAM Broth, 500 g
7670T PALCAM Broth, 5 kg