Peptone Water

Peptone Water is used for the cultivation of non-fastidious microorganisms, indole testing, and as a basal medium for carbohydrate fermentation studies in a laboratory setting. Peptone Water is not intended for use in the diagnosis of disease or other conditions in humans.

Peptone Water is a minimal growth medium. The formulation of Peptone Water permits cultivation of non-fastidious organisms. This non-selective medium has been used as a basal medium for biochemical tests such as carbohydrate fermentation patterns and production of indole. A 1% final carbohydrate concentration with phenol red (0.018 g/L) indicator and a Durham tube can be used for fermentation studies.


Formula Liter
Peptone 10 g
Sodium Chloride 5 g

Final pH: 7.2 ± 0.2 at 25°C
Formula may be adjusted and/or supplemented as required to meet performance specifications.


  1. Dissolve 15 g of the medium in one liter of purified water.
  2. Mix thoroughly.
  3. Autoclave at 121°C for 15 minutes.

Order Details

Product # Product Description
7365A Peptone Water, 500 g
7365T Peptone Water, 5 kg