PPLO Broth without CV

PPLO Broth without CV is used with enrichments for the isolation and cultivation of Mycoplasma spp. in a laboratory setting. PPLO Broth without CV is not intended for use in the diagnosis of disease or other conditions in humans.

PPLO Broth without CV is prepared according to the formula described by Morton and Lecce. Crystal Violet may inhibit the growth of Mycoplasma, and therefore was removed from an earlier formula described by Morton, Smith, Williams, and Eikenberg. Mycoplasma was discovered in a case of pleuropneumonia in a cow, and referred to as "pleuropneumonia-like organism" or PPLO. Mycoplasmas belong to the class of Mollicutes "soft skin", which are the smallest free-living organisms. They are pleomorphic, varying in size from 0.2 to 0.3 micromillimeters.


Formula Liter
Heart Infusion 6 g
Yeast Enriched Peptone 10 g
Sodium Chloride 5 g

Final pH: 7.8 ± 0.2 at 25°C
Formula may be adjusted and/or supplemented as required to meet performance specifications.


Sterile Serum Supplement, 300 mL


  1. Dissolve 21 g of the medium in 700 mL of purified water.
  2. Mix thoroughly.
  3. Autoclave at 121°C for 15 minutes.
  4. Cool to 45 – 50°C and aseptically add 300 mL of the appropriate sterile serum supplement.
  5. Mix thoroughly.

Order Details

Product # Product Description
7485A PPLO Broth without CV, 500 g
7485T PPLO Broth without CV, 5 kg