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Pseudomonas Agar Base

Pseudomonas Agar Base is used for the selective and differential isolation of Pseudomonas sp. using CN or CFC supplementation in a laboratory setting. Pseudomonas Agar Base is not intended for use in the diagnosis of disease or other conditions in humans.

Pseudomonas Agar Base was developed to be supplemented with CN (Cetrimide & Sodium Nalidixate) or CFC (Cetrimide, Fucidin, & Cephaloridine). This base formula is a modification of King's A Medium. The CN Supplement is recommended for the isolation of Pseudomonas aeruginosa, where the addition of Sodium Nalidixate and the reduction of Cetrimide improved recovery. Pseudomonas Agar Base with the addition of CN Supplement, demonstrated enhanced pigment formation of P. aeruginosa and increased inhibition of KlebsiellaProteus, and Providencia spp.

Pseudomonas Agar Base with the addition of CFC Supplement is recommended for the selective isolation of Pseudomonas spp. Mead and Adams reduced Cetrimide, permitting growth of all pigmented and non-pigmented psychrophilic pseudomonads. The antimicrobics, Fucidin and Cephaloridine, were added to increase the selectivity of the medium. This formulation is used for Pseudomonas spp. from chilled foods and processing plants. Burkholderia cepacia (formerly Ps. cepacia) is isolated from water sources, and can be problematic in the preparation of sterile products for the pharmaceutical, food, and cosmetic industries. Pseudomonas Agar Base is recommended by ISO for the enumeration of Pseudomonasspp. from meat and meat products.

 

Formula Liter
Gelatin Peptone 16 g
Enzymatic Digest of Casein 10 g
Potassium Sulfate 10 g
Magnesium Chloride 1.4 g
Agar 11 g

Final pH: 7.1 ± 0.2 at 25°C
Formula may be adjusted and/or supplemented as required to meet performance specifications.

Supplement /Liter

10 mL Glycerol

CN Supplement

200 mg/L Cetrimide
15 mg/L Sodium Nalidixate

CFC Supplement

10 mg/L Cetrimide
10 mg/L Fucidin
50 mg/L Cephaloridine

Directions

  1. Dissolve 48.4 g of the medium and 10 mL of Glycerol in one liter of purified water and bring to a boil.
  2. Autoclave at 121°C for 15 minutes.
  3. Cool to 45 – 50C before adding supplements.

CN Formula: Add 200 mg/L Cetrimide and 15 mg/L Sodium Nalidixate.
CFC Formula: Add 10 mg/L Cetrimide, 10 mg/L Fucidin and 50 mg/L Cephaloridine.
Note: Dissolve supplements with 2 mL ethanol and 2 mL deionized water; filter sterilize.

Order Details

Product # Product Description
9222A Pseudomonas Agar Base, 500 g
9222C Pseudomonas Agar Base, 10 kg