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Pseudomonas Isolation Agar

Pseudomonas Isolation Agar is used for the isolation of Pseudomonas aeruginosa and other Pseudomonas spp. in a laboratory setting. Pseudomonas Isolation Agar is not intended for use in the diagnosis of disease or other conditions in humans.

Pseudomonas Isolation Agar is based on Medium A described by King, Ward, and Raney. This medium is very useful for isolating Pseudomonas spp. Pseudomonas Isolation Agar includes Irgasan, a potent broad-spectrum antimicrobial not active against Pseudomonas spp. This medium is selective and formulated to enhanced formation of blue or blue-green pyocyanin pigment by P. aeruginosa. The pigment diffuses into the medium surrounding growth.

 

Formula Liter
Enzymatic Digest of Gelatin 20 g
Magnesium Chloride 1.4 g
Potassium Sulfate 10 g
Irgasan® 0.025 g
Agar 13.6 g

Final pH: 7.0 ± 0.2 at 25°C
Formula may be adjusted and/or supplemented as required to meet performance specifications.

Supplement / Liter

Glycerol, 20 mL

Directions

  1. Suspend 45 g of the medium in one liter of purified water containing 20 mL of glycerol.
  2. Heat with frequent agitation and boil for one minute to completely dissolve the medium.
  3. Autoclave at 121°C for 15 minutes.

Order Details

Product # Product Description
7329A Pseudomonas Isolation Agar, 500 g
7329T Pseudomonas Isolation Agar, 5 kg