Rappaport-Vassiliadis (MSRV) Medium Semisolid Modified

Rappaport-Vassiliadis (MSRV) Medium Semisolid Modified is used with Novobiocin for the rapid detection of motile Salmonella spp. in a laboratory setting. Rappaport-Vassiliadis (MSRV) Medium Semisolid Modified is not intended for use in the diagnosis of disease or other conditions in humans.

Rappaport-Vassiliadis (MSRV) Medium Semisolid Modified is a modification of Rappaport-Vassiliadis enrichment broth for detecting motile Salmonella spp. in feces and food products. The original research on MSRV Medium revealed a semisolid could be used as a rapid and sensitive test for isolating motile Salmonella from food products following pre-enrichment or selective enrichment. The semisolid medium allows motility to be detected as halos of growth around the original point of inoculation.

MSRV Medium is recommended by the European Chocolate Manufacturer's Association. A collaborative study performed with support of American Cocoa Research Institute and the Canadian Chocolate Manufacturer's Association resulted in first action adoption of the MSRV method by AOAC International. MSRV Medium may be used as a plating medium for isolating Salmonella spp. (other than S. typhi and S. partyphi type A) from stool specimens with high sensitivity and specificity.


Formula Liter
Enzymatic Digest of Casein 4.59 g
Casein Acid Hydrolysate 4.59 g
Sodium Chloride 7.34 g
Potassium Dihydrogen Phosphate 1.47 g
Magnesium Chloride, Anhydrous 10.93 g
Malachite Green Oxalate 0.037 g
Agar 2.7

Final pH: 7.2 ± 0.2 at 25°C
Formula may be adjusted and/or supplemented as required to meet performance specifications.

Novobiocin Supplement

Novobiocin, 20 mg


  1. Suspend 31.6 g of the medium in one liter of purified water.
  2. Heat with frequent agitation and boil for one minute to completely dissolve the medium.
  4. Cool medium to 45 – 50°C and aseptically add 10 mL of Novobiocin Supplement.
  5. Mix well and dispense into Petri dishes.

Order Details

Product # Product Description
7511A Rappaport-Vassiliadis (MSRV) Medium Semisolid Modified, 500 g
7511T Rappaport-Vassiliadis (MSRV) Medium Semisolid Modified, 5 kg