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Rappaport-Vassiliadis R10 Broth

Rappaport-Vassiliadis R10 Broth is used for the selective enrichment of Salmonella spp. from foods in a laboratory setting. Rappaport-Vassiliadis R10 Broth is not intended for use in the diagnosis of disease or other conditions in humans.

Rappaport et al. formulated an enrichment medium for Salmonella spp. that was modified by Vassiliadis et al. The Rappaport formulation, designated R 25/37°C, recommended incubation at 37°C. The Vassiliadis modification, designated R 10/43°C, had a reduced level of Malachite Green and recommended incubation at 43°C. Peterz later showed that incubation at 41.5 ± 0.5°C for 24 hours improved recovery of Salmonella spp. Rappaport-Vassiliadis R10 Broth is a selective enrichment medium that is used following pre-enrichment of the specimen. It has gained approval for use in analyzing milk and milk products, raw flesh foods, highly contaminated foods, and animal feeds. This medium selectively enriches for Salmonella spp. because bacteria, including other intestinal bacteria, are typically susceptible to or inhibited by Malachite Green, high osmotic pressure and/or low pH. S. typhi and S. choleraesuis are sensitive to Malachite Green and may be inhibited.

 

Formula Liter
Enzymatic Digest of Casein 4.54 g
Sodium Chloride 7.20 g
Potassium Dihydrogen Phosphate 1.45 g
Magnesium Chloride, Anhydrous 13.4 g
Malachite Green Oxalate 0.036 g

Final pH: 5.1 ± 0.2 at 25°C
Formula may be adjusted and/or supplemented as required to meet performance specifications.

Directions

  1. Dissolve 26.6 g of the medium in one liter of purified water.
  2. Mix thoroughly.
  3. Autoclave at 116°C (10 lb pressure) for 15 minutes.

Order Details

Product # Product Description
7512A Rappaport-Vassiliadis R10 Broth, 500 g
7512T Rappaport-Vassiliadis R10 Broth, 5 kg