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Rappaport-Vassiliadis Salmonella Enrichment Broth

Rappaport-Vassiliadis Salmonella Enrichment Broth is used for the enrichment and selective isolation of Salmonella spp. Conforms to Harmonized USP/EP/JP Requirements in a laboratory setting. Rappaport-Vassiliadis Salmonella Enrichment Broth is not intended for use in the diagnosis of disease or other conditions in humans.

Rappaport et al formulated an enrichment medium for Salmonella spp. that was modified by Vassiliadis et al. The Rappaport formulation, designated R 25/37°C, recommended incubation at 37°C. The Vassiliadis modification, designated R 10/43°C, had a reduced level of Malachite Green and recommended incubation at 43°C. Peterz later showed that incubation at 41.5 ± 0.5°C for 24 hours improved recovery of Salmonella spp.

Rappaport-Vassiliadis Salmonella Enrichment Broth is a modification of Rappaport-Vassiliadis R10 Broth, where Soy Peptone has replaced Enzymatic Digest of Casein as the nitrogen and vitamin source. Soy Peptone has shown to enhance the growth of Salmonella spp. and minimizes Bovine Spongiform Encephalopathy (BSE) risk. Rappaport-Vassiliadis Salmonella Enrichment Broth conforms to Harmonized United States Pharmacopoeia (USP), European Pharmacopoeia (EU), and Japanese Pharmacopoeia (JP). This medium selectively enriches for Salmonella spp. because bacteria, including other intestinal bacteria, are typically susceptible to or inhibited by Malachite Green, high osmotic pressure and/or low pH. S. typhi and S. choleraesuis are sensitive to Malachite Green and may be inhibited.

 

Formula Liter
Soy Peptone 4.5 g
Sodium Chloride 8 g
Potassium Phosphate, monobasic 0.6 g
Potassium Phosphate, dibasic 0.4 g
Magnesium Chloride, anhydrous* 13.58 g
Malachite Green 0.036 g

  *Equivalent to 29.0 g/L Magnesium Chloride hexahydrate
Final pH: 5.0 ± 0.2 at 25°C
Formula may be adjusted and/or supplemented as required to meet performance specifications.

Directions

  1. Dissolve 27.2 g of the medium in one liter of purified water.
  2. Mix thoroughly.
  3. Dispense 10 mL into glass tubes, cap and autoclave at 115°C for 15 minutes.

Order Details

Product # Product Description
7730A Rappaport-Vassiliadis Salmonella Enrichment Broth, 500 g
7730T Rappaport-Vassiliadis Salmonella Enrichment Broth, 5 kg