Reinforced Clostridial Medium

Reinforced Clostridial Medium is used for the cultivation of anaerobes from food and clinical specimens. Conforms to Harmonized USP/EP/JP Requirements in a laboratory setting. Reinforced Clostridial Medium is not intended for use in the diagnosis of disease or other conditions in humans.

Reinforced Clostridial Medium is an enriched, non-selective medium formulated by Hirsch and Grinstead. This medium was developed for the isolation of spore-forming anaerobes, especially Clostridium spp. The developers demonstrated the medium was more fertile and enabled growth to be initiated from small inocula more readily than five other media tested. Hirsch and Grinstead added Polymyxin B, 15 – 20 mg/L, to inhibit the growth of Gram-negative microorganisms.

Reinforced Clostridial Medium conforms to Harmonized United States Pharmacopoeia (USP), European Pharmacopoeia (EU), and Japanese Pharmacopoeia (JP).


Formula Liter
Beef Extract 10 g
Peptone 10 g
Sodium Chloride 5 g
Dextrose 5 g
Yeast Extract 3 g
Sodium Acetate 3 g
Soluble Starch 1 g
L-Cysteine HCI 0.5 g
Agar 0.5 g

Final pH: 6.8 ± 0.2 at 25°C
Formula may be adjusted and/or supplemented as required to meet performance specifications.


  1. Suspend 38 g of the medium in one liter of purified water.
  2. Heat with frequent agitation to completely dissolve the medium.
  3. Autoclave at 121°C for 15 minutes.

Order Details

Product # Product Description
7714A Reinforced Clostridial Medium, 500 g
7714C Reinforced Clostridial Medium, 10 kg