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Rose Bengal Chloramphenicol Agar

Rose Bengal Chloramphenicol Agar is used for the selective isolation and enumeration of yeasts and molds from foods in a laboratory setting. Rose Bengal Chloramphenicol Agar is not intended for use in the diagnosis of disease or other conditions in humans.

Rose Bengal Chloramphenicol Agar is a selective medium for the enumeration of fungi. In 1944, Smith and Dawson used Rose Bengal for the selective isolation of fungi from soil samples. This formula was prepared with a neutral pH, deviating from the usual acidified medium. Several investigations have found a neutral pH with the addition of a selective agent have been successful in supporting fungal growth and restricting bacterial growth. Rose Bengal Agar supplemented with Chloramphenicol is a modification of the Rose Bengal Chlortetracycline Agar formula of Jarvis. Chloramphenicol is recommended as the selective agent in fungal medium with a neutral pH because of its heat stability and broad antibacterial spectrum.

Rose Bengal Chloramphenicol Agar is recommended in standard methods for the enumeration of yeast and molds from foods and water. Rose Bengal Chloramphenicol Agar is also referred to as Rose Bengal Agar and Rose Bengal-Malt Extract Agar.

 

Formula Liter
Enzymatic Digest of Soybean Meal 5 g
Dextrose 10 g
Monopotassium Phosphate 1 g
Magnesium Sulfate 0.5 g
Rose Bengal 0.05 g
Chloramphenicol 0.1 g
Agar, Bacteriological 15.5 g

Final pH: 7.2 ± 0.2 at 25°C
Formula may be adjusted and/or supplemented as required to meet performance specifications.

Directions

  1. Dissolve 32.2 grams of the medium in one liter of purified water.
  2. Heat with frequent agitation and boil for one minute to completely dissolve the medium.
  3. Autoclave at 121°C for 15 minutes.

Order Details

Product # Product Description
7664A Rose Bengal Chloramphenicol Agar, 500 g
7664T Rose Bengal Chloramphenicol Agar, 5 kg