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Sabouraud Dextrose Agar with Chloramphenicol

Sabouraud Dextrose Agar with Chloramphenicol is used for the selective isolation of fungi in a laboratory setting. Sabouraud Dextrose Agar with Chloramphenicol is not intended for use in the diagnosis of disease or other conditions in humans.

Sabouraud Dextrose Agar (SDA) is a modification of Dextrose Agar described by Sabouraud. Sabouraud Dextrose Agar is used for cultivating pathogenic and commensal fungi and yeasts. The high dextrose concentration and acidic pH of the formula permits selectivity of fungi. This medium is beneficial in sporulation studies and pigment production. Sabouraud Dextrose Agar is used for determining the microbial content of cosmetics and in the mycological evaluation of food.

Sabouraud Dextrose Agar with Chloramphenicol is a modification of Sabouraud Dextrose Agar, with the addition of Chloramphenicol to increase selectivity against commensal microorganisms.

 

Formula Liter
Enzymatic Digest of Casein 5 g
Enzymatic Digest of Animal Tissue 5 g
Dextrose 40 g
Chloramphenicol 0.05 g
Agar 15 g

Final pH: 5.6 ± 0.2 at 25°C
Formula may be adjusted and/or supplemented as required to meet performance specifications.

Directions

  1. Suspend 65 g of the medium in one liter of purified water.
  2. Heat with frequent agitation and boil for one minute to completely dissolve the medium.
  3. Autoclave at 121°C for 15 minutes.

Order Details

Product # Product Description
7306A Sabouraud Dextrose Agar with Chloramphenicol, 500 g
7306T Sabouraud Dextrose Agar with Chloramphenicol, 5 kg