Sabouraud Dextrose Broth

Sabouraud Dextrose Broth is used for the cultivation of fungi from sterile and non-sterile products in a laboratory setting. Sabouraud Dextrose Broth is not intended for use in the diagnosis of disease or other conditions in humans. Conforms to Harmonized USP/EP/JP Requirements.

Sabouraud Dextrose Broth is a modification of Dextrose Agar described by Sabouraud. Sabouraud Dextrose Media are used for cultivating pathogenic & commensal fungi and yeasts. The high dextrose concentration and acidic pH of the formulas permit selectivity of fungi. Sabouraud Dextrose Broth is used for the determination of fungistatic activity in sterile and non-sterile food & beverage, and cosmetic products.

Sabouraud Dextrose Broth conforms to Harmonized United States Pharmacopoeia (USP), European Pharmacopoeia (EU), and Japanese Pharmacopoeia (JP).


Formula Liter
Enzymatic Digest of Casein 5 g
Enzymatic Digest of Animal Tissue 5 g
Dextrose 20 g

Final pH: 5.6 ± 0.2 at 25°C
Formula may be adjusted and/or supplemented as required to meet performance specifications.


  1. Dissolve 30 g of the medium in one liter of purified water.
  2. Mix thoroughly.
  3. Autoclave at 121°C for 15 minutes.

Order Details

Product # Product Description
7617A Sabouraud Dextrose Broth, 500 g
7617T Sabouraud Dextrose Broth, 5 kg