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Schaedler Agar

Schaedler Agar is used for the cultivation of anaerobic microorganisms in a laboratory setting. Schaedler Agar is not intended for use in the diagnosis of disease or other conditions in humans.

Survival of anaerobic bacteria is dependent on their sensitivity to oxygen, nutritional requirements, appropriate collection, culture medium, and incubation time and temperature. Schaedler Agar is suitable for standard procedures used in cultivating anaerobic bacteria.

Schaedler Agar is prepared according to the formulation described by Schaedler, Dubos, and Costello, and modified by Mata, Carrillo, and Villatoro. Modifications include reduced dextrose to avoid interference with hemolytic reactions, reduced yeast extract to avoid darkening of the medium, and adjusted sodium chloride and nitrogen concentrations.

 

Formula Liter
Tryptic Soy Broth 10 g
Enzymatic Digest of Casein 2.5 g
Enzymatic Digest of Animal Tissue 2.5 g
Yeast Extract 5 g
Dextrose 5 g
Tris (hydroxymethyl) Aminomethane 3 g
Hemin 0.01 g
L-Cystine 0.4 g
Agar 13.5 g

Final pH: 7.6 ± 0.2 at 25°C
Formula may be adjusted and/or supplemented as required to meet performance specifications.

Directions

  1. Suspend 41.9 g of the medium in one liter of purified water.
  2. Heat with frequent agitation and boil for one minute to completely dissolve the medium.
  3. Autoclave at 121°C for 15 minutes.

Order Details

Product # Product Description
7153A Schaedler Agar, 500 g
7153T Schaedler Agar, 5 kg