Selenite Broth

Selenite Broth is used for the selective enrichment of Salmonella spp. in a laboratory setting. Selenite Broth is not intended for use in the diagnosis of disease or other conditions in humans.

Selenite Broth was originated by Leifson, while observing good recovery of Salmonella spp. and reduced growth of fecal coliforms. Salmonella organisms are also injured in food-processing procedures, including exposure to low temperatures, sub-marginal heat, drying, radiation, preservatives or sanitizers.

Selenite Broth conforms with the American Public Health Association (APHA). Many modifications of Selenite Broth exist, including Selenite Cystine Broth, from the original formula described as Selenite F Broth by Leifson.


Formula Liter
Enzymatic Digest of Casein 2.5 g
Enzymatic Digest of Animal Tissue 2.5 g
Lactose 4 g
Sodium Phosphate 10 g
Sodium Selenite 4 g

Final pH: 7.0 ± 0.2 at 25°C
Formula may be adjusted and/or supplemented as required to meet performance specifications.


  1. Dissolve 23 g of the medium in one liter of purified water.
  2. Heat to boiling. Avoid overheating.

Order Details

Product # Product Description
7155A Selenite Broth, 500 g
7155T Selenite Broth, 5 kg