TAT Broth

TAT Broth is used for the detection of microorganisms in cosmetics and topical drugs in a laboratory setting. TAT Broth is not intended for use in the diagnosis of disease or other conditions in humans.

TAT Broth is prepared for use in the examination of cosmetics and topical drugs. TAT Broth, with the addition of Polysorbate 20, is recommended for sterility testing of viscous materials, including salves or ointments. It is especially adapted for sterility testing of cosmetics. Cosmetic products are subject to contamination during manufacturing and use by consumers. Preservatives are used in aqueous products to make them self-sterilizing for vegetative bacteria, yeast, and molds.

TAT (Tryptone-Azolectin-Tween) Broth is also referred to as Fluid Casein Digest-Soy Lecithin Polysorbate 20 Medium.


Formula Liter
Enzymatic Digest of Casein 20 g
Lecithin 5 g

Final pH: 7.2 ± 0.2 at 25°C
Formula may be adjusted and/or supplemented as required to meet performance specifications.


Polysorbate 20, 40 mL


  1. Suspend 25 g of the medium in 960 mL of purified water.
  2. Add 40 mL of Polysorbate 20 to the suspended medium.
  3. Place the mixture in a 48 – 50°C water bath for 30 minutes. Stir occasionally.
  4. Autoclave at 121°C for 15 minutes.

Order Details

Product # Product Description
7219A TAT Broth, 500 g
7219T TAT Broth, 5 kg