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Terrific Broth, Modified

Terrific Broth, Modified is used with glycerol in cultivating recombinant strains of Escherichia coli in a laboratory setting. Terrific Broth, Modified is not intended for use in the diagnosis of disease or other conditions in humans.

Terrific Broth, Modified was developed by Tartoff and Hobbs to increase yields of plasmid DNA from transformed E. coli. Terrific Broth, Modified is a popular choice of media available for plasmid preps. While cells grown in Terrific Broth, Modified can grow slower than other liquid media, the final cell density and plasmid yields are 2 – 4 fold higher. The higher yield is a result of the increased concentration of Enzymatic Digest of Casein and Yeast Extract in this formula. Glycerol is used as a carbohydrate source, and unlike glucose, is not fermented to acetic acid.

 

Formula Liter
Yeast Extract 24 g
Enzymatic Digest of Casein 12 g
Dipotassium Phosphate 9.4 g
Monopotassium Phosphate 2.2 g

Final pH: 7.2 ± 0.2 at 25°C
Formula may be adjusted and/or supplemented as required to meet performance specifications.

Supplement

Glycerol, 8 mL

Directions

  1. Dissolve 47.6 g of the medium in one liter of purified water containing 8 mL of glycerol.
  2. Mix thoroughly.
  3. Autoclave at 121°C for 15 minutes.

Order Details

Product # Product Description
7708A Terrific Broth, Modified, 500 g
7708T Terrific Broth, Modified, 5 kg