Tetrathionate Broth Base, Hajna

Tetrathionate Broth Base, Hajna is used a supplement with for the isolation of Salmonella spp. (except S. typhi) from samples in a laboratory setting. Tetrathionate Broth Base, Hajna is not intended for use in the diagnosis of disease or other conditions in humans.

Tetrathionate Broth Base, Hajna is used as a selective enrichment for the cultivation of Salmonella spp. Salmonella organisms may be present but injured in food-processing procedures, which include exposure to low temperatures, sub-marginal heat, drying, radiation, preservative, and sanitizers. From May 1 to November 30, 2010, over 19000 reported cases of Salmonella Enteritidis (SE) infections were associated with contaminated shell eggs. SE infections can be very serious and symptoms can included diarrhea, fever, abdominal cramps, headache, nausea and vomiting. Eggs can become contaminated on the farm because a laying hen can become infected with SE and pass the bacteria into the egg before it is laid. If the egg is not refrigerated, the bacteria can grow inside the uncracked, whole egg. Tetrathionate Broth Base, Hajna, is recommended in the FDA/BAM Method for the Environmental Sampling and Detection of Salmonella Enteritidis (SE) in Poultry Houses and the Compendium of Methods for the Microbiological Examination of Foods.

Tetrathionate Broth Base, Hajna was developed by Hajna and Damon.


Formula Liter
Sodium Thiosulfate 38 g
Calcium Carbonate 25 g
Casein/Meat Peptone (50:50) 18 g
Sodium Chloride 5 g
D-Mannitol 2.5 g
Yeast Extract 2 g
Glucose 0.5 g
Sodium Deoxycholate 0.5 g
Brilliant Green 0.01 g

Final pH: 7.6 ± 0.2 at 25°C
Formula may be adjusted and/or supplemented as required to meet performance specifications.


Iodine-Potassium Iodide Solution Composition per 40.0 mL:
KI, 8 g
Iodine, 5 g


  1. Suspend 91.5 grams of the medium in 960 milliliters of purified water.
  2. Heat with frequent agitation and boil for one minute to completely dissolve the medium.
  3. DO NOT AUTOCLAVE. Cool to 40 – 45°C.
  4. Just prior to inoculation, add 40 mL of Iodine/Potassium Iodide Solution to the prepared Tetrathionate Broth Base, Hajna.
  5. Distribute into sterile capped containers in 10 mL volumes.
    Note: Do not add Iodine/Potassium Iodide Solution to tubes until just before inoculation. Chemical Tetrathionate inhibits by oxidation of Thiosulfate through the addition of Iodine just prior to use.

Order Details

Product # Product Description
7740A Tetrathionate Broth Base, Hajna, 500 g
7740T Tetrathionate Broth Base, Hajna, 5 kg