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Tetrathionate Broth Base

Tetrathionate Broth Base is used with iodine for the recovery of Salmonella spp. in a laboratory setting. Tetrathionate Broth Base is not intended for use in the diagnosis of disease or other conditions in humans.

Tetrathionate Broth Base is used as a selective enrichment for the cultivation of Salmonella spp. Salmonella organisms may be injured in food-processing procedures, which include exposure to low temperatures, sub-marginal heat, drying, radiation, preservative, and sanitizers.

Mueller demonstrated the effectiveness of Tetrathionate Broth for enriching typhoid and paratyphoid bacilli while inhibiting coliform organisms. Using modified Mueller's broth, Kauffmann increased the number of positive isolates. Tetrathionate Broth was used in studies for the poultry industry and in a collaborative study for rapid screening of Salmonella in food. Tetrathionate Broth Base, abbreviated as TT Broth Base, is specified in standard methods for Salmonella testing. The FDA, Bacteriological Analytical Manual incorporate Tetrathionate Broth Base as a pre-enrichment medium for detecting Salmonella in food materials.

 

Formula Liter
Enzymatic Digest of Casein 2.5 g
Enzymatic Digest of Animal Tissue 2.5 g
Bile Salts 1 g
Calcium Carbonate 10 g
Sodium Thiosulfate 30 g

Final pH: 8.4 ± 0.2 at 25°C
Formula may be adjusted and/or supplemented as required to meet performance specifications.

Supplement

Iodine-Potassium Iodide Solution
(Composition per 20.0 mL: KI – 5.0 g, Iodine – 6.0 g)

Directions

  1. Dissolve 46 g of the medium in one liter of purified water.
  2. Heat with frequent agitation to boiling.
  3. Cool to 45°C and add 20 mL of the Iodine-Potassium Iodide Solution to the prepared Tetrathionate Broth Base. If preparing solution, add 6 grams Iodine + 5 grams Potassium Iodide in 20 mL of purified water.
  4. DO NOT REHEAT AFTER ADDING IODINE SOLUTION.
    Note: Do not add Iodine/Potassium Iodide Solution to tubes until just before inoculation. Chemical Tetrathionate inhibits by oxidation of Thiosulfate through the addition of Iodine just prior to use

Order Details

Product # Product Description
7241A Tetrathionate Broth Base, 500 g
7241T Tetrathionate Broth Base, 5 kg