Thioglycollate Medium without Indicator

Thioglcyollate Medium without Indicator is used for the cultivation of anaerobic microorganisms in a laboratory setting. Thioglcyollate Medium is not intended for use in the diagnosis of disease or other conditions in humans.

Quastel and Stephenson found the presence of small amounts of a compound containing an –SH group (cysteine, thioglycollic acid, and glutathione) permitted "aerobic" growth of Clostridium sporogenes. Falk, Bucca, and Simmons discovered the advantages of using small quantities of agar in detecting contaminants during sterility testing. Brewer demonstrated the value of a small amount of agar and a reducing substance in this medium.

Thioglycollate Medium without Indicator is used for cultivating and detecting microorganisms in normally sterile materials, especially those containing mercurial preservatives when the oxidation-reduction indicator is not present or required. Thioglcyollate Medium without Indicator is the medium of choice for diagnostic testing, where lack of an indicator avoids possible toxicity to organisms.

Final pH: 7.0 ± 0.2 at 25°C
Formula may be adjusted and/or supplemented as required to meet performance specifications.

Directions

1. Dissolve 29.5 g of the medium in one liter of purified water.
2. Heat with frequent agitation to completely dissolve the medium.
3. Autoclave at 121°C for 15 minutes. Cool to room temperature.

NOTE: The prepared medium should be reduced prior to inoculation. The prepared tubes should be boiled (with caps loose) for 3 – 5 minutes and cooled before use. Alternatively, the tubes can be placed in an anaerobic environment for at least 3 hours before use.

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