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Todd Hewitt Broth

Todd Hewitt Broth is used for the cultivation of streptococci and other fastidious microorganisms in a laboratory setting. Todd Hewitt Broth is not intended for use in the diagnosis of disease or other conditions in humans.

Todd Hewitt Broth was originally developed for the production of antigenic streptococcal hemolysin. Todd Hewitt Broth is prepared according to the formula described by Updyke and Nickle, who compared media for type specific extract production of group A streptococci. This study was performed using Todd Hewitt Broth prepared with infusion of fresh beef heart as a control. Results showed Todd Hewitt Broth was particularly satisfactory for growth of group A streptococci for serological typing.

Todd Hewitt Broth is recommended as an enrichment medium for growth of streptococcal cells in the identification of groups A and B by IF staining. Todd Hewitt Broth was used as an enrichment broth for group A streptococci in a comparison study of a rapid antigen test.

 

Formula Liter
Heart Infusion (dehydrated) 3.1 g
Yeast Enriched Peptone 20 g
Dextrose 2 g
Sodium Chloride 2 g
Disodium Phosphate 0.4 g
Sodium Carbonate 2.5 g

Final pH: 7.8 ± 0.2 at 25°C
Formula may be adjusted and/or supplemented as required to meet performance specifications.

Directions

  1. Dissolve 30 g of the medium in one liter of purified water.
  2. Heat with frequent agitation to completely dissolve the medium.
  3. Autoclave at 121°C for 15 minutes.

Order Details

Product # Product Description
7161A Todd Hewitt Broth, 500 g
7161T Todd Hewitt Broth, 5 kg