Tomato Juice Agar

Tomato Juice Agar is used for the cultivation of lactobacilli.

In 1925, Mickle and Breed reported the use of tomato juice in culture media used for cultivating lactobacilli. Kulp investigated tomato juice on bacterial development and found growth of L. acidophilus was enhanced. Tomato Juice Agar, prepared according to Kulp and White's modification, is especially useful in cultivating L. acidophilus from clinical specimens and foodstuffs.


Formula Liter
Tomato Juice Solids 20 g
Enzymatic Digest of Casein 10 g
Peptonized Milk 10 g
Agar 11 g

Final pH: 6.1 ± 0.2 at 25°C
Formula may be adjusted and/or supplemented as required to meet performance specifications.


  1. Suspend 51 g of the medium in one liter of purified water.
  2. Heat with frequent agitation and boil for one minute to completely dissolve the medium.
  3. Autoclave at 121°C for 15 minutes.

Order Details

Product # Product Description
7349A Tomato Juice Agar, 500 g
7349T Tomato Juice Agar, 5 kg