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Tryptic Soy Agar with Lecithin & Tween 80

Tryptic Soy Agar with Lecithin & Tween 80 is used for the isolation of microorganisms from surfaces sanitized with quaternary ammonium compounds in a laboratory setting. Tryptic Soy Agar with Lecithin & Tween 80 is not intended for use in the diagnosis of disease or other conditions in humans.

In 1955, Leavitt et al. discovered Tryptic Soy Agar supported excellent growth of aerobic and anaerobic microorganisms. Tryptic Soy Agar is a nutritious base and a variety of supplements are added to enhance the medium, including Lecithin and Tween 80. The Lecithin and Tween 80 inactivate some preservatives that may inhibit bacterial growth, reducing "preservative carryover". Tryptic Soy Agar with Lecithin & Tween 80 is recommended for determining the sanitation efficiency of containers, equipment, and work area (environmental monitoring).

 

Formula Liter
Enzymatic Digest of Casein 15 g
Enzymatic Digest of Soybean Meal 5 g
Sodium Chloride 5 g
Lecithin 0.7 g
Tween 80 5 g
Agar 20.5 g

Final pH: 7.3 ± 0.2 at 25°C
Formula may be adjusted and/or supplemented as required to meet performance specifications.

Directions

  1. Suspend 51.2 g of the medium in one liter of purified water.
  2. Heat with frequent agitation and boil for one minute to completely dissolve the medium.
  3. Autoclave at 121°C for 15 minutes.

Order Details

Product # Product Description
7163A Tryptic Soy Agar with Lecithin & Tween 80, 500 g
7163T Tryptic Soy Agar with Lecithin & Tween 80, 5 kg