Tryptic Soy Broth with Ferrous Sulfate

Tryptic Soy Broth with Ferrous Sulfate is used in the pre-enrichment of Salmonella for shell and liquid whole eggs as described in FDA/Bacteriological Analytical Manual1 in a laboratory setting. Tryptic Soy Broth with Ferrous Sulfate is not intended for use in the diagnosis of disease or other conditions in humans.

Tryptic Soy Broth is a general purpose medium used for the cultivation of a wide variety of microorganisms, and conforms to Harmonized USP/EP/JP Requirements. Tryptic Soy Broth, is commonly referred to as Soybean-Casein Digest Medium, and abbreviated as TSB. Tryptic Soy Broth complies with established standards in the food industry.

Tryptic Soy Broth with Ferrous Sulfate is used in the FDA/Bacteriological Analytical Manual (BAM) Method for the Identification and Isolation of Salmonella in Eggs. TSB with Ferrous Sulfate is used in the Pre-Enrichment Step for Shell Eggs and Liquid Whole Eggs (Homogenized). Egg-associated illness caused by Salmonella is a serious public health problem. Salmonella enteritidis has been implicated in egg-associated illness, and can cause abdominal cramps, fever, and diarrhea beginning 12 to 72 hours after consuming a contaminated food or beverage. Salmonella enteritidis can be found inside eggs that appear normal. Eggs in the shell become contaminated on the farm, primarily because of an infection in the laying hens.


Formula Liter
Enzymatic Digest of Casein 17 g
Enzymatic Digest of Soybean Meal 3 g
Sodium Chloride 5 g
Dipotassium Phosphate 2.5 g
Dextrose 2.5 g
Ferrous Sulfate 0.035 g

Final pH: 7.3 ± 0.2 at 25°C
Formula may be adjusted and/or supplemented as required to meet performance specifications.


  1. Suspend 30 g of the medium in one liter of purified water.
  2. Autoclave at 121°C for 15 minutes.

Order Details

Product # Product Description
7739A Tryptic Soy Broth with Ferrous Sulfate, 500 g