Home


 

Tryptic Soy Broth, Modified with Acid Digest of Casein

Tryptic Soy Broth, Modified with Acid Digest of Casein is used for the selective enrichment of enterohemorrhagic E. coli in foods in a laboratory setting. Tryptic Soy Broth, Modified with Acid Digest of Casein is not intended for use in the diagnosis of disease or other conditions in humans. Conforms to USDA Formulation with Acid Digest of Casein.

The first major outbreak of E. coli O157:H7 was in 1982, and traced to contaminated hamburgers. Other known sources of infection include sprouts, lettuce, salami, unpasteurized milk, juice and/or swimming in or drinking contaminated water. Tryptic Soy Broth, Modified with Acid Digest of Casein is used to enrich food samples suspected of having low levels of EHEC during pathogen testing.

 

Formula Liter
Enzymatic Digest of Casein 17 g
Acid Digest of Casein 10 g
Sodium Chloride 5 g
Dipotassium Phosphate 4 g
Enzymatic Digest of Soybean Meal 3 g
Dextrose 2.5 g
Bile Salts No. 3 1.5 g

Final pH: 7.3 ± 0.2 at 25°C
Formula may be adjusted and/or supplemented as required to meet performance specifications.

Directions

  1. Suspend 43 grams of the medium in 1000 mL of purified water.
  2. Autoclave at 121°C for 15 minutes.
  3. If required, add filter sterilized Novobiocin solution at desired concentration (adjusted for 100% potency).

Order Details

Product # Product Description
7167A Tryptic Soy Broth, Modified with Acid Digest of Casein, 500 g
7167T Tryptic Soy Broth, Modified with Acid Digest of Casein, 5 kg