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Tryptic Soy Broth, Modified with Novobiocin

Tryptic Soy Broth, Modified with Novobiocin is used for the selective enrichment of enterohemorrhagic E. coli in foods in a laboratory setting. Tryptic Soy Broth, Modified with Novobiocin is not intended for use in the diagnosis of disease or other conditions in humans.

The first major outbreak of E. coli O157:H7 was in 1982, and traced to contaminated hamburgers. Other known sources of infection include sprouts, lettuce, salami, unpasteurized milk, juice and/or swimming in or drinking contaminated water.

Tryptic Soy Broth, Modified with Novobiocin is used to enrich food samples suspected of having low levels of EHEC during pathogen testing.

 

Formula Liter
Enzymatic Digest of Casein 17 g
Sodium Chloride 5 g
Dipotassium Phosphate 4 g
Enzymatic Digest of Soybean Meal 3 g
Dextrose 2.5 g
Bile Salts No. 3 1.5 g
Novobiocin 0.020 g

Final pH: 7.3 ± 0.2 at 25°C
Formula may be adjusted and/or supplemented as required to meet performance specifications.

Directions

  1. Dissolve 33 g of the medium in one liter of purified water.
  2. Autoclave at 121°C for 15 minutes.

Order Details

Product # Product Description
7694A Tryptic Soy Broth, Modified with Novobiocin, 500 g
7694T Tryptic Soy Broth, Modified with Novobiocin, 5 kg