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Tryptic Soy Broth, Modified with 20 mg Novobiocin & Acid Digest of Casein

Tryptic Soy Broth, Modified with 20 mg Novobiocin & Acid Digest of Casein is used for the selective enrichment of enterohemorrhagic E. coli in foods. Conforms to USDA formulation with Novobiocin supplementation in a laboratory setting. Tryptic Soy Broth, Modified with 20 mg Novobiocin & Acid Digest of Casein is not intended for use in the diagnosis of disease or other conditions in humans.

The first major outbreak of E. coli O157:H7 was in 1982, and traced to contaminated hamburgers. Other known sources of infection include sprouts, lettuce, salami, unpasteurized milk, juice and/or swimming in or drinking contaminated water. Tryptic Soy Broth, Modified with 20 mg Novobiocin & Acid Digest of Casein is used to enrich food samples suspected of having low levels of EHEC during pathogen testing.

 

Formula Liter
Enzymatic Digest of Casein 17 g
Acid Digest of Casein 10 g
Sodium Chloride 5 g
Dipotassium Phosphate 4 g
Enzymatic Digest of Soybean Meal 3 g
Dextrose 2.5 g
Bile Salts No. 3 1.5 g
Novobiocin 0.02 g

Final pH: 7.3 ± 0.2 at 25°C
Formula may be adjusted and/or supplemented as required to meet performance specifications.

Directions

  1. Suspend 43.02 g of the medium in 1000 mL of purified water.
  2. Autoclave at 121°C for 15 minutes.

Order Details

Product # Product Description
7731A Tryptic Soy Broth, Modified with 20 mg Novobiocin & Acid Digest of Casein, 500 g