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Tryptone Glucose Extract Agar

Tryptone Glucose Extract Agar is used for the cultivation and enumeration of microorganisms in dairy and bottled water products in a laboratory setting. Tryptone Glucose Extract Agar is not intended for use in the diagnosis of disease or other conditions in humans.

In the 1930's, Bower and Hucker developed a medium for detecting bacteria in milk and other dairy products. Prickett used a glucose agar containing tryptone to study thermophilic bacteria in milk. This medium is known as Yeast Dextrose Agar. In 1948, the American Public Health Association (APHA) adopted Tryptone Glucose Extract Agar for use in testing milk and dairy products. Currently, APHA specifies Tryptone Glucose Extract Agar for the heterotrophic plate count procedure in testing bottled water.

Tryptone Glucose Extract Agar is also known as Yeast Dextrose Agar.

 

Formula Liter
Beef Extract 3 g
Enzymatic Digest of Casein 5 g
Dextrose 1 g
Agar 15 g

Final pH: 7.0 ± 0.2 at 25°C
Formula may be adjusted and/or supplemented as required to meet performance specifications.

Directions

  1. Suspend 24 g of the medium in one liter of purified water.
  2. Heat with frequent agitation and boil for one minute to completely dissolve the medium.
  3. Autoclave at 121°C for 15 minutes.

Order Details

Product # Product Description
7242A Tryptone Glucose Extract Agar, 500 g
7242T Tryptone Glucose Extract Agar, 5 kg