Tryptose Broth

Tryptose Broth is used for the cultivation of Brucella spp. and other fastidious microorganisms in a laboratory setting. Tryptose Broth is not intended for use in the diagnosis of disease or other conditions in humans.

Tryptose Broth is prepared with Tryptose, and recommended for the cultivation and isolation of pathogenic and saprophytic bacteria. Historically, it was considered necessary to include meat extract or infusion as a nutritional supplement in culture media. Tryptose was developed while studying growth requirements of Brucella spp. Huddleson found Tryptose media to be equal or superior to meat infusion media, providing uniformity for the cultivation and differentiation of fastidious organisms.

Tryptose Broth can be used as a complete basal medium or supplemented with enrichment. Huddleson used a broth containing 2% Tryptose as an enrichment medium in the isolation of Brucella spp. from samples. McCullough et al. reported that addition of thiamine, dextrose, and iron salts increased growth of Brucella suis. Addition of 0.1% agar to Tryptose Broth can increase growth of aerobes and anaerobes in liquid media. Tryptose media are recommended in standard methods for food testing.


Formula Liter
Tryptose 20 g
Sodium Chloride 5 g
Dextrose 1 g

Final pH: 7.2 ± 0.2 at 25°C
Formula may be adjusted and/or supplemented as required to meet performance specifications.


  1. Dissolve 26 g of the medium in one liter of purified water.
  2. Mix thoroughly.
  3. Autoclave at 121°C for 15 minutes.

Order Details

Product # Product Description
7367A Tryptose Broth, 500 g
7367T Tryptose Broth, 5 kg