Tryptose Phosphate Broth

Tryptose Phosphate Broth is used for the cultivation of a wide variety of fastidious microorganisms in a laboratory setting. Tryptose Phosphate Broth is not intended for use in the diagnosis of disease or other conditions in humans.

Tryptose Phosphate Broth is an infusion-free buffered medium recommended for the cultivation of fastidious, pathogenic microorganisms.

Tryptose Phosphate Broth is valuable in tissue culture procedures, as shown by Ginsberg, Gold, and Jordan. The proteose content of Tryptose Phosphate Broth is considered to be a stimulating factor for cells. Tryptose Phosphate Broth is specified for cell culture procedures.


Formula Liter
Enzymatic Digest of Casein 20 g
Dextrose 2 g
Sodium Chloride 5 g
Disodium Phosphate 2.5 g

Final pH: 7.3 ± 0.2 at 25°C
Formula may be adjusted and/or supplemented as required to meet performance specifications.


  1. Dissolve 29.5 g of the medium in one liter of purified water.
  2. Mix thoroughly.
  3. Autoclave at 121°C for 15 minutes.

Order Details

Product # Product Description
7278A Tryptose Phosphate Broth, 500 g
7278T Tryptose Phosphate Broth, 5 kg