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Universal Pre-Enrichment Broth

Universal Pre-Enrichment Broth is used for the recovery of Salmonella spp. and Listeria spp. in a laboratory setting. Universal Pre-Enrichment Broth is not intended for use in the diagnosis of disease or other conditions in humans.

Traditional methods for recovering Salmonella spp. and Listeria spp. from food products require separate pre-enrichment media for each microorganism. Some broth media recommended for pre-enrichment contain antibiotic inhibitors, or have insufficient buffering capacity which hinder recovery of sublethally injured cells.

Bailey and Cox formulated Universal Pre-Enrichment Broth to permit simultaneous resuscitation of sublethally injured Salmonella and Listeria. The broth medium provides sufficient buffering capacity to prevent rapid decreases in pH, and allows for repair of injured cells that might be sensitive to low pH values or inhibitory substances.

Universal Pre-Enrichment Broth is used as the pre-enrichment for Salmonella testing of orange & apple juice, cider, cantaloupes, tomatoes, and mamey pulp according to FDA/BAM Methods.

 

Formula Liter
Enzymatic Digest of Casein 5 g
Proteose Peptone 5 g
Potassium Phosphate Monobasic 15 g
Sodium Phosphate Dibasic 7 g
Sodium Chloride 5 g
Dextrose 0.5 g
Magnesium Sulfate 0.25 g
Ferric Ammonium Citrate 0.1 g
Sodium Pyruvate 0.2 g

Final pH: 6.3 ± 0.2 at 25°C
Formula may be adjusted and/or supplemented as required to meet performance specifications.

Directions

  1. Dissolve 38 g of the medium in one liter of purified water.
  2. Heat with frequent agitation to completely dissolve the medium.
  3. Autoclave at 121°C for 15 minutes.

Order Details

Product # Product Description
7510A Universal Pre-Enrichment Broth, 500 g
7510T Universal Pre-Enrichment Broth, 5 kg