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UVM Modified Listeria Enrichment Broth

UVM Modified Listeria Enrichment Broth is used for the selective enrichment of Listeria spp. in a laboratory setting. UVM Modified Listeria Enrichment Broth is not intended for use in the diagnosis of disease or other conditions in humans.

UVM Modified Listeria Enrichment Broth is a modification of the formula described by Donnelly and Baigent. This formula is used for the selective enrichment of Listeria spp. from food. Listeria spp. grow over a pH range of 5.0 – 9.6, and survive in food products with pH levels outside these parameters. Listeria spp. are microaerophilic, Gram-positive, asporogenous, non-encapsulated, non-branching, short, motile rods. Motility is pronounced at 20°C. Identification of Listeria is based on successful isolation of the organism, biochemical characterization, and serological confirmation.

 

Formula Liter
Enzymatic Digest of Casein 5 g
Enzymatic Digest of Animal Tissue 5 g
Beef Extract 5 g
Yeast Extract 5 g
Sodium Chloride 20 g
Disodium Phosphate 9.6 g
Monopotassium Phosphate 1.35 g
Esculin 1 g
Acriflavin 0.012 g
Nalidixic Acid 0.02 g

Final pH: 7.2 ± 0.2 at 25°C
Formula may be adjusted and/or supplemented as required to meet performance specifications.

Directions

  1. Dissolve 52 g of the medium in one liter of purified water.
  2. Mix thoroughly.
  3. Autoclave at 121°C for 15 minutes.

Order Details

Product # Product Description
7409A UVM Modified Listeria Enrichment Broth, 500 g
7409T UVM Modified Listeria Enrichment Broth, 5 kg