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Violet Red Bile Agar with MUG

Violet Red Bile Agar with MUG is used for the detection of coliforms and the fluorogenic detection of Escherichia coli in a laboratory setting. Violet Red Bile Agar with MUG is not intended for use in the diagnosis of disease or other conditions in humans.

Violet Red Bile Agar with MUG is specified in many procedures to enumerate coliforms in food and dairy products. In 1982, Feng and Hartman developed a rapid fluorogenic assay for Escherichia coli by incorporating 4-methylumbelliferyl-β-D-glucuronide (MUG) into Lauryl Tryptose Broth. Incorporating MUG into Violet Red Bile Agar permits the detection of E. coliamong the coliform colonies. Standard methods procedures specify Violet Red Bile Agar with MUG for detecting E. coli in food and dairy products by fluorescence. This medium is often abbreviated as VRBA w/ MUG.

 

Formula Liter
Enzymatic Digest of Gelatin 7 g
Yeast Extract 3 g
Bile Salts Mixture 1.5 g
Lactose 10 g
Sodium Chloride 5 g
Neutral Red 0.03 g
Crystal Violet 0.002 g
4-Methylumbelliferyl-β-D-Glucuronide 0.1 g
Agar 15 g

Final pH: 7.4 ± 0.2 at 25°C
Formula may be adjusted and/or supplemented as required to meet performance specifications.

Directions

  1. Suspend 41.6 g of the medium in one liter of purified water.
  2. Heat with frequent agitation and boil for only two minutes to completely dissolve the medium.
  3. Cool the medium to 45 – 46°C and pour plates.

Order Details

Product # Product Description
7359A Violet Red Bile Agar with MUG, 500 g
7359B Violet Red Bile Agar with MUG, 2 kg
7359T Violet Red Bile Agar with MUG, 5 kg