W-L Nutrient Medium

W-L Nutrient Medium is used for the cultivation of yeasts, molds, and bacteria encountered in brewing and industrial fermentations in a laboratory setting. W-L Nutrient Medium is not intended for use in the diagnosis of disease or other conditions in humans.

W-L Nutrient Medium was developed by Green and Gray while studying various fermentation processes. An exhaustive study examining methods of fermentation control procedures in worts, beers, liquid yeasts and similar fermentation products led to the development of W-L Nutrient Medium. At a pH of 5.5, counts of viable baker's yeast will grow on W-L Nutrient Medium.

W-L Nutrient Medium is also referred to as "Wallerstein Laboratory Medium".


Formula Liter
Yeast Extract 4 g
Enzymatic Digest of Casein 5 g
Dextrose 50 g
Monopotassium Phosphate 0.55 g
Potassium Chloride 0.425 g
Calcium Chloride 0.125 g
Magnesium Sulfate 0.125 g
Ferric Chloride 0.0025 g
Manganese Sulfate 0.0025 g
Bromcresol Green 0.022 g
Agar 20 g

Final pH: 5.5 ± 0.2 at 25°C
Formula may be adjusted and/or supplemented as required to meet performance specifications.


  1. Dissolve 80 g of the medium in one liter of purified water.
  2. Heat with frequent agitation to completely dissolve the medium.
  3. Autoclave at 121°C for 15 minutes.

Order Details

Product # Product Description
7488A W-L Nutrient Medium, 500 g
7488T W-L Nutrient Medium, 5 kg