Home


 

Wilkins-Chalgren Agar

Wilkins-Chalgren Agar is used for the cultivation of anaerobic microorganisms in a laboratory setting. Wilkins-Chalgren Agar is not intended for use in the diagnosis of disease or other conditions in humans.

This medium was selected because it does not require the addition of blood to support satisfactory growth of most anaerobes. The survival of anaerobic bacteria is dependent on their sensitivity to oxygen, nutritional requirements, appropriate collection, culture medium, and incubation time and temperature.

 

Formula Liter
Enzymatic Digest of Casein 10 g
Enzymatic Digest of Gelatin 10 g
Yeast Extract 5 g
Sodium Chloride 5 g
Dextrose 1 g
L-Arginine 1 g
Sodium Pyruvate 1 g
Hemin 0.005 g
Vitamin K 0.0005 g
Agar 15 g

Final pH: 7.1 ± 0.2 at 25°C
Formula may be adjusted and/or supplemented as required to meet performance specifications.

Directions

  1. Suspend 48 g of the medium in one liter of purified water.
  2. Heat with frequent agitation and boil for one minute to completely dissolve the medium.
  3. Autoclave at 121°C for 15 minutes.

Order Details

Product # Product Description
7232A Wilkins-Chalgren Agar, 500 g
7232T Wilkins-Chalgren Agar, 5 kg