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YM-11 Agar

YM-11 Agar is used for the selective enumeration of yeasts and molds using the ISO-GRID® and/or NEO-GRID® Membrane Filtration System. YM-11 Agar is not intended for use in the diagnosis of disease or other conditions in humans.

YM-11 Agar was formulated to stimulate rapid growth and colonial development of a wide range of fungi. Peptone sources were selected to maximize growth is this medium. YM-11 Agar is buffered at a neutral pH, and selective agents were included to inhibit bacteria growth.

YM-11 Agar is recommended for the rapid enumeration of yeasts and molds in all foods using the ISO-GRID and/or NEO-GRID Membrane Filtration System method.

 

Formula Liter
Enzymatic Digest of Soybean Meal 20 g
Enzymatic Digest of Casein 20 g
Dextrose 5 g
Sodium Chloride 5 g
Potassium Phosphate, Dibasic 2.4 g
Trypan Blue 0.03 g
Chloramphenoicol 0.1 g
Agar 15 g

Final pH: 7.0 ± 0.2 at 25°C
Formula may be adjusted and/or supplemented as required to meet performance specifications.

Supplement

Presterilized Antibiotic Supplement, Chlortetracycline-HCl, 20 mL:
To reconstitute, aseptically add 100 mL sterile distilled water to a bottle containing 0.5 g pre-sterilized powder and shake to dissolve. Store Chlortetracycline solution at 2 - 8°C for up to 60 days.

Directions

  1. Suspend 67.5 g of the medium in one liter of purified water.
  2. Heat with frequent agitation and boil for one minute to completely dissolve the medium.
  3. Autoclave at 121° C for 15 minutes.
  4. Cool to 45 – 50°C. Aseptically add 20 mL of a 0.5 A% (wt/v) aqueous solution of Chlortetracycline HCl. Mix thoroughly.
  5. Check pH and adjust, if necessary to obtain a final pH of 7.0 ± 0.2 in the solidified medium.
  6. Dispense 18 – 20 mL volumes to sterile Petri dishes, cool until plates are solidified.

Order Details

Product # Product Description
6904A YM-11 Agar, 500 g