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YM Agar

YM Agar is used for the cultivation of yeasts, molds, and other aciduric organisms in a laboratory setting. YM Agar is not intended for use in the diagnosis of disease or other conditions in humans.

YM Agar is prepared according to the formulation published by Wickerham. YM Agar was formulated for the selective isolation of yeasts from mixed cultures containing bacteria and molds. Media selectivity may be enhanced through acidification or through addition of selective agents. YM Agar should be sterilized without pH adjustment and sterile acid added to the medium cooled to 45 – 50°C. Acidified YM Agar should not be heated. Antibiotics may be aseptically added to sterile media. Other fungistatic materials may be added to YM Agar to eliminate molds and permit enumeration of yeasts to mixed populations.

 

Formula Liter
Enzymatic Digest of Gelatin 5 g
Yeast Extract 3 g
Malt Extract 3 g
Dextrose 10 g
Agar 20 g

Final pH: 6.2 ± 0.2 at 25°C
Formula may be adjusted and/or supplemented as required to meet performance specifications.

Directions

  1. Suspend 41 g of the medium in one liter of purified water.
  2. Heat with frequent agitation and boil for one minute to completely dissolve the medium.
  3. Autoclave at 121°C for 15 minutes.

Order Details

Product # Product Description
7525A YM Agar, 500 g
7525T YM Agar, 5 kg