Bacteria, Yeast and Fungi Identification

Biolog's latest generation redox chemistry enables testing and microbial identification of aerobic Gram-negative and Gram-positive bacteria in the same test panel. Gram stain and other pre-tests are no longer needed. A simple one minute setup protocol and your samples are ready to be analyzed. Our expanded GEN III database is designed to meet the needs of Biolog's broad customer base covering diverse disciplines of microbiology. All Biolog Microbial Identification Systems — manual, semi-automated or fully automated — use the powerful new GENIII MicroPlate, allowing users to determine the most appropriate system to fit their current budget and level of throughput. Should needs change, all systems can be upgraded and expanded to meet new capacity requirements.

Biolog’s range of identification products includes:

- GENIII: Aerobic Gram Negative and Gram Positive Identification
- AN: Anaerobic Identification
- YT: Yeast Identification
- FF: Filamentous Fungi Identification
- MT2: User Defined MicroPlate
- SF-N2 & SF-P2: Sporulating and Filamentous Microorganisms

GENIII: Aerobic Gram Negative and Gram Positive Identification

The new GENIII redox chemistry is applicable to an unprecedented range of both gram negative and gram positive bacteria. GENIII detects and analyzes the ability of the cell to metabolize all major classes of biochemicals, in addition to determining other important physiological properties such as pH, salt, and lactic acid tolerance, reducing power, and chemical sensitivity. Identifications can be performed manually, or with all Biolog instruments including the semi-automated MicroStation™ and the automated OmniLog®.

AN: Anaerobe Identification

The Biolog AN MicroPlate TM performs 95 discrete tests simultaneously and gives a characteristic reaction pattern called a “metabolic fingerprint”. These fingerprint reaction patterns provide a vast amount of information conveniently contained on a single Biolog MicroPlate TM. The patterns are compared using Biolog MicroLogTM software and database to provide an identification.

YT: Yeast Identification

The Biolog YT MicroPlate™ is designed for identification and characterization of a very wide range of Yeasts. Biolog’s MicroPlates and databases were first introduced in 1989, employing a novel, patented redox chemistry. This chemistry, based on reduction of tetrazolium, responds to the process of metabolism (i.e. respiration) rather than to metabolic by-products (e.g. acid). Biolog’s chemistry works as a universal reporter of metabolism and simplifies the testing process as color developing chemicals do not need to be added. Since the YT MicroPlate™ measures both metabolic reactions as well as turbidity growth to produce identifications, it provides superior capability for all types of yeasts organisms

FF: Filamentous Fungi Identification

The FF MicroPlate employs a redox chemistry similar to Biolog's other proven microbial identification/characterization products. This chemistry, based on reduction of tetrazolium, responds to the process of metabolism (oxidation of substrates). Biolog’s universal chemistry works with any carbon source and greatly simplifies the testing process, as no color developing chemicals need to be added after incubation. The FF database also analyzes fungal growth via turbidimetric analysis.

MT2: User Defined MicroPlate

The MT2 MicroPlateTM (Biolog Cat. # 1013) provides a standardized micromethod for performing up to 95 carbon source utilization tests in a single panel. The user has complete flexibility in selecting the carbon sources and in configuring the tests within the panel (for example by row or by column). Biolog’s MicroLogTM 2 or MicroLogTM 3 software may be used to construct a customized database to identify or compare specific strains based on their metabolic patterns in user-defined MT2 MicroPlatesTM.

SF-N2 & SF-P2 MicroPlate

The SF-N2 and SF-P2 MicroPlates can be used for easy and rapid metabolic testing of Sporulating and Filamentous (SF) microorganisms such as actinomycetes and fungi. Traditionally, testing of sporulating and filamentous microbes has been very labor intensive, difficult, and slow. Because they can form hydrophobic mycelial filaments, these organisms have a strong tendency to form clumps and adhere to surfaces, making them difficult to handle. Furthermore, because they can form spores, germinate, and then undergo complex life cycles, it is very difficult to get cultures which perform consistently in metabolic and biochemical testing regimes.