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GENIII: Aerobic Gram Negative and Gram Positive Identification

The GENIII MicroPlate test panel provides a standardized micromethod using 94 biochemical tests to profile and identify a broad range of Gram-negative and Gram-positive bacteria. Biolog’s Microbial Identification Systems software is used to identify the bacterium from its phenotypic pattern in the GEN III MicroPlate.

The 94 phenotypic tests include 71 carbon source utilization assays and 23 chemical sensitivity assays. The test panel provides a “Phenotypic Fingerprint” of the microorganism that can be used to identify it at the species level. All necessary nutrients and biochemicals are prefilled and dried into the 96 wells of the MicroPlate. Tetrazolium redox dyes are used to colorimetrically indicate utilization of the carbon sources or resistance to inhibitory chemicals.

Following inoculation of the wells, which start out colorless, there is increased respiration during incubation in the wells where cells can utilize a carbon source and/or grow. Increased respiration causes reduction of the tetrazolium redox dye, forming a purple color. Negative wells remain colorless, as does the negative control well with no carbon source. There is also a positive control well used as a reference for the chemical sensitivity assays. After incubation, the phenotypic fingerprint of purple wells is compared to Biolog’s extensive species library, either manually, or on one of the Biolog instruments. If a match is found, a species level identification of the isolate is made.

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