Testing of SF microorganisms in SF-N2 and SF-P2 MicroPlates is very simple and uses virtually the same protocol and equipment used for testing coccoid and rod-shaped bacteria and yeast in the MicroLogTM System. The four basic steps are virtually the same, but with a few simple modifications. Special culture media are used for these microorganisms to promote sporulation. Inocula are prepared at a lower density, approximately one tenth the density normally used. Inocula are prepared in a gel-forming colloid instead of in water or saline. The suspensions are inoculated at 100µl per well (instead of 150µl per well). The SF-N2 and SF-P2 MicroPlates do not contain the tetrazolium redox dye which is toxic to many of these species. After inoculation, the SF MicroPlates are typically incubated at an appropriate low temperature (e.g. 26°C) for several days (e.g. 1 to 5).
The inoculated microorganism grows in wells containing carbon sources that it can utilize, forming cloudy turbidity in those wells as compared to the A-1 reference well. The colloid used to suspend the cells enhances growth and keeps the cells uniformly suspended. After sufficient incubation, “positive” reactions are determined as increased cloudiness or turbidity. Many of these microorganisms also produce distinct characteristics such as pigments in wells with particular carbon sources.
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