Bile Salts Mixture No. 3 is a mixture of bile salts for use in preparing microbiological culture media in a laboratory setting. Bile Salts Mixture No. 3 is not intended for use in the diagnosis of disease or other conditions in humans.
Bile Salts Mixture No. 3 is a mixture of sodium cholate and sodium deoxychollate. Its primary use is as a selective agent for the isolation of Gram-negative microorganisms, inhibiting Gram-positive cocci. Bile Salts Mixture No. 3 is prepared especially for use in MacConkey Agar and Violet Red Bile Agar. Bile Salts Mixture No. 3 is soluble in distilled or deionized water and neutral to slightly alkaline in pH. It is used in preparing culture media without pre-filtration or adjustment of reaction. Several media containing Bile Salts Mixture No. 3 are recommended in standard methods for multiple applications.