Campylobacter Supplement (Bolton's)

Campylobacter Supplement (Bolton's) is used with Campylobacter Enrichment Broth (Bolton's) for the selective enrichment of Campylobacter spp. in food and water samples. Campylobacter Supplement (Bolton's) is not intended for use in the diagnosis of disease or other conditions in humans.


Formula (per vial)
Cefoperazone 10 mg
Vancomycin 10 mg
Trimethoprim 10 mg
Cycloheximide 25 mg


  1. Dissolve 27.6 g of the medium in one liter of purified water.
  2. Allow powder to soak for 10 minutes.
  3. Heat with frequent agitation to completely dissolve the medium.
  4. Autoclave at 121°C for 15 minutes.
  5. Cool medium to 45 – 50°C and if desired, aseptically add 50 mL of lysed horse blood and 2 vials of reconstituted Campylobacter Supplement (Bolton's) (# 7998).