Harlequin® Cronobacter sakazakii Isolation Medium is used for the isolation of Enterobacter sakazakii from milk and milk product as recommended under ISO/TS 22964:2006(E).
Harlequin CSIM was developed to isolate and differentiate Cronobacter sakazakii (formerly Enterobacter sakazakii), a member of the Enterobacteriaceae family. C. sakazakii is differentiated when it hydrolyses the chromogenic substrate 5-bromo-4-chloro-3-indolyl-α-D-glucopyranoside present in the medium. Sodium deoxycholate and crystal violet were included in the formula to make the medium more selective.
1. Weigh 29.75 grams of powder and disperse in 1 liter of deionized water.
2. Allow to soak for 10 minutes, swirl to mix
3. Autoclave for 15 minutes at 121°C.
4. Cool to 47°C and mix well before dispensing into Petri dishes.
|Pancreatic peptone of casein||7 g|
|Yeast extract||3 g|
|Sodium chloride||5 g|
|Sodium deoxycholate||0.6 g|
|Crystal violet||0.002 g|
|Grams per liter||29.75 g|
Final pH: 7.0 ± 0.2