Listeria Selective Diagnostic Supplement

A selective diagnostic supplement containing cycloheximide, nalidixic acid and phosphatidylinositol for the isolation and presumptive identification of Listeria monocytogenes. For use with HAL010 Harelquin™ Listeria Chromogenic Agar.


Formula (per vial) X010 X210
Cycloheximide 25 mg 125 mg
Nalidixic acid 10 mg 50 mg
Phosphatidylinositol ~300 mg ~1500 mg


Pre-heat X010/X210 to 48-50°C and aseptically add to sterilised medium cooled to 48-50°C.

  1. Add 1 vial X010 and 1 vial of X072 to 475 mL medium OR
  2. Add 1 vial of X210 and 5 vials of X072 to 237 5mL medium