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Novobiocin Supplement

Novobiocin Supplement is used with culture media as a supplement for the selective enrichment of Salmonella spp. and coliforms and E. coli and selects against susceptible Staphylococci spp. in a laboratory setting. Novobiocin Supplement is not intended for use in the diagnosis of disease or other conditions in humans.

 

Formula (per vial)
Novobiocin (Sodium salt) 20 mg

Directions

MSRV:

  1. Suspend 31.6 g of the medium in one liter of purified water.
  2. Heat with frequent agitation and boil for one minute to completely dissolve the medium.
  3. DO NOT AUTOCLAVE.
  4. Cool medium to 45 – 50°C and aseptically add 10 mL of Novobiocin Supplement (7985).
  5. Mix well and dispense into Petri dishes.

EC Medium, Modified:

  1. Dissolve 36.6 g of the medium in one liter of purified water.
  2. Mix thoroughly.
  3. Autoclave at 121°C for 15 minutes.
  4. Cool to room temperature and add 10 mL of the Novobiocin Supplement (7985).
  5. Dispense aseptically into sterile tubes containing an inverted fermentation Durham tube.

Tryptic Soy Broth Modified w/ Acid Digest of Casein

  1. Suspend 43 grams of the medium in 1000 mL of purified water.
  2. Autoclave at 121°C for 15 minutes.
  3. Cool to room temperature and add 10 mL of the Novobiocin Supplement (7985).