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PALCAM Supplement

PALCAM Supplement is used with PALCAM Agar or PALCAM Broth as a supplement for the selective and differential enrichment of Listeria spp. from foods, dairy and environmental samples in a laboratory setting. PALCAM Supplement is not intended for use in the diagnosis of disease or other conditions in humans.

 

Formula (per vial)
Polymyxin B 5 mg
Acriflavine 2.5 mg
Ceftazidime 10 mg

Directions

PALCAM Agar Base:

  1. Suspend 72 g of the medium in one liter of purified water.
  2. Heat with frequent agitation and boil for one minute to completely dissolve the medium.
  3. Autoclave at 121°C for 15 minutes. Cool to 45 – 50°C.
  4. Aseptically add 10 mL of PALCAM Supplement (# 7987). One vial will supplement 500 mL of PALCAM Agar Base.
  5. Dispense into sterile Petri dishes.

PALCAM Broth:

  1. Suspend 47 g of the medium in one liter of purified water.
  2. Heat with frequent agitation to completely dissolve the medium.
  3. Autoclave at 121°C for 15 minutes. Cool to 45 – 50°C.
  4. Aseptically add 10 mL of PALCAM Supplement (# 7987). One vial of PALCAM Supplement will supplement 500 mL of PALCAM Broth.
  5. Dispense into sterile tubes.