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Polymyxin B Supplement

Polymyxin B Supplement is used with culture media as a supplement for the selective enrichment of Bacillus spp. in a laboratory setting. Polymyxin B Supplement is not intended for use in the diagnosis of disease or other conditions in humans.

 

Formula (per vial)
Polymyxin B 50,000 IU

Directions

MYP Agar Base:

  1. Suspend 23 g of the medium in 450 mL of purified water.
  2. Heat with frequent agitation and boil for one minute to completely dissolve the medium.
  3. Autoclave at 121°C for 15 minutes.
  4. Cool to 45 – 50°C
  5. Aseptically supplement with 50 mL Egg Yolk Emulsion (7982) and 5 mL of a 50,000 IU Polymyxin B solution (7997).

Bacillus cereus Agar Base:

  1. Suspend 41 g of the medium in 950 mL of purified water.
  2. Heat with frequent agitation and boil for one minute to completely dissolve the medium.
  3. Autoclave at 121°C for 15 minutes.
  4. Cool to 45 – 50°C.
  5. Aseptically add 50 mL of Egg Yolk Emulsion (# 7982) and 10 mL of Polymyxin B (# 7997, 2 x 5 mL vials)