Tryptic Soy Broth

Tryptic Soy Broth is used for the cultivation of a wide variety of microorganisms in a laboratory setting. Tryptic Soy Broth is not intended for use in the diagnosis of disease or other conditions in humans. Tryptic Soy Broth conforms to Harmonized USP/EP/JP Requirements.

Tryptic Soy Broth, a general purpose medium, is commonly referred to as Soybean-Casein Digest Medium, and abbreviated as TSB. This medium was originally developed for use without blood in determining the effectiveness of sulfonamides against pneumococci and other organisms. Clostridia and non-sporulating anaerobes grow luxuriantly in this broth when incubated under anaerobic conditions. TSB is recommended for testing bacterial contaminants in cosmetics and complies with established standards in the food industry. TSB was chosen by the USDA Animal and Plant Health Inspection Service for detecting viable bacteria in live vaccines.

TSB conforms to Harmonized United States Pharmacopoeia (USP), European Pharmacopoeia (EU), and Japanese Pharmacopoeia (JP). With the addition of 6.5% NaCl, TSB can be used for the selective growth of group D streptococci.


Formula Liter
Enzymatic Digest of Casein 17 g
Enzymatic Digest of Soybean Meal 3 g
Sodium Chloride 5 g
Dipotassium Phosphate 2.5 g
Dextrose 2.5 g

Final pH: 7.3 ± 0.2 at 25°C
Formula may be adjusted and/or supplemented as required to meet performance specifications.


  1. Add 375 g sample to sample bag.
  2. Add contents of Acumedia EZ Prep packet to sample bag.
  3. Add 3.375 L sterile purified water.
  4. Mix thoroughly (a stomacher can be used).